Note: This is not a full list of all fluorochromes that are compatible with the flow cytometers. Phase III+: The University is open for expanded research operations; only authorized personnel will be admitted on campus. Copyright 2004—2020 The Rockefeller University. All rights reserved. Contact, Flow Cytometry Resource Center (FCRC) The default configuration of the BD Accuri® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. Easy to use - fixed alignment and pre-optimized detector settings. Also the filters and lasers can be swapped out to suit your needs with easily available off-the-shelf substitutes (this was mentioned by one of the Accuri techs, I personally cannot verify this). Brand: BD 653156 69 0 obj <>stream ... BD Accuri C6. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. Click on “View” tab 4. Data is digitally collected over a 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. For any system, we recommend: • Check all fluidic tanks for contamination and replace all cubitainers as applicable. BD offers a selction of inferior vena cava filter products. The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. Cat. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. The Selectable Lasers Module allows operation of the system in two additional configurations (2-Blue 2-Red, and 4-Blue) and significantly expands the fluorochrome combinations that can be analyzed. A compact optical design, fixed alignment, and pre- BD Accuri™ C6 software was started. BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. h�b``�```�����`��π ���@q��L�n\g���`��h 'c�. FCRC Waiting List Forms A unique, non-pressurized, peristaltic pump system drives the fluidics. 661085). The BD Accuri C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. BD LSRFortessa X-20. Table of Contents. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. BD LSRFortessa 1. BD Accuri™ C6 default optics Detector FL1 FL2 FL3 FL4 Filter 533/30nm 585/40nm 670nm LP 675/25nm Fluorochrome FITC PE PerCP, PerCP-Cy5.5 APC Color Green Yellow/Orange Dark Red Red “3 blue 1 … The ideal cell concentration The Accuri C6 cytometer in my lab has some problems, and we need some help. To view hidden file extensions which distinguish BD Accuri C6 Software files (.c6) from BD Accuri C6 Software template files (.c6t): 1. 0 BD Accuri™ C6 Flow Cytometer Features: Equipped with a blue and a red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. Rockefeller University 1230 York Avenue, Box 344 Brand BD Accuri™ Contents Contains (12) Peristaltic Pump Tubing, (6) Sheath Bottle Filters, (12) Decon/Cleaner Bottle Filters and (6) In-Line Sheath Filters The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. Click on the Windows Start button and select Control Panel from the pop-up menu. BD Accuri C6, C6 Plus, and BD FACSVia Cytometers Perform these procedures when starting up your instrument after an extended period. No. You are viewing an Accuri C6 Flow Cytometer. A unique low-pressure pumping system drives the fluidics. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration of over 5 x 106 cells per mL. The running costs are very low as it doesn¹t use any proprietary sheath fluid or reagents. Overall very good cosmetic condition. 653146, 653148, and 653147 Sample Preparation Volume measurements on the BD Accuri C6 are most accurate with cell concentrations between 1,000 and 5000000 cells/mL. Higher concentrations might result in inaccurate counting due to system saturation, so dilute samples if necessary. This Technical Note Amnis ImageStreamX. BD LSRFortessa 2. C6 Flow Cytometer 1-year Maintenance Kit . The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. 2. A compact optical design, fixed This item has been powered on, but no further testing. BD Accuri® C6 CFlow SAMPLER supports the high throughput and fully automated acquisition from 48- and 96-well plates and deep-well plates. Since yeasts are relatively large (I use an Accuri C6 to count bacteria so yeast are large to me) I would go with the faster/larger core size. Filter and Fluorochrome List for the Accuri® C6 Flow Cytometer® System ©January, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP The BD CSampler Plus adds a minimal footprint to the BD Accuri C6 Plus, about three feet square for the pair, keeping your benchtop free for other uses. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. New York, NY 10065. The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. BD™ Accuri™ Bacteriostatic Concentrate Solution can be used to treat 10L of sheath fluid. Hi, our Accuri C6 has stopped taking liquid. Such wear includes scratches, scrapes, and general dirtiness. Which fluorochromes are compatible with the cytometers? A compact optical design. Online Booking System -Ģ �r^�F)�?2��7�% �K��x4JR���E�Y��Wl������3� �;p�y[�AD= r Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. Ē\�@BBH"v@��d� �Hz �H4�0012����H%�?�ݏ x�S To increase signal resolution, or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable filters is available. filter, and bottle filters. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. Even more colors to choose from with selectable lasers and filter replacement. … Select “Tools” from the menu bar. Came with note that it needs repair. ... optical filters optimized for the detection of FITC, PE, PerCP, and APC. Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. Access to training videos presented by FCR staff are here. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. 52 0 obj <>/Filter/FlateDecode/ID[<6D17E613E92D6948AEF4F9A1572DFD51>]/Index[29 41]/Info 28 0 R/Length 106/Prev 60895/Root 30 0 R/Size 70/Type/XRef/W[1 2 1]>>stream BD Accuri C6 Flow Cytometer Instrument Manual 7820018 Rev-4 1 1 INTRODUCTION The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. With minor signs of normal wear and tear. Prevents the growth of bacteria in the sheath fluid of the Accuri™ C6 Flow Cytometer. BD Accuri® C6 is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. %PDF-1.6 %���� 29 0 obj <> endobj As with any fluorochrome used in a flow cytometric assay, the success of FP detection depends on the level of FP expression within the cells, the excitation wavelength used and the light filters placed in front of the fluorescence detectors. Accuri C6 is a digital PC based bench top analyser, 2 laser system. hެ�mS�8ǿ�^��[���L��@H�]�LC�w���$J�c����O�R�B ��:aGZ�V��6h���eZ3.ӆ !�N��Ӗ).�Θ����Y�2��V2#W�%㙱�(&���h&2�OäV 3��b#�(!5{���W�֕78���L�/��� Gը�_���~=ZU0�-a��aPW�Cw�����W8v4 �•���r���֍qlj�Į��!��e���N�f��p�c��ޟ-[ޟ�0��͵��I�~F�������ѭ3��w0�C�z�����?~������S�d��r��>U�j���~1���U#�^�&L�˥ӥM�����R)拷���. Training can be requested via PPMS. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). ... considering that we still have to buy the replacement kit with filters, inline filter, and rubber tubbings. Performance validation of the BD Accuri C6 … Clinical Research and the Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience Science, the Arts, and Culture. the Accuri C6 Flow Cytometer System. You can also set custom speeds/core sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. Available to trained users with online booking. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible In addition, the system derives sample volume and can calculate absolute counts or sample concentration per microliter. Millex Sterile syringe filter units are available in a larger 33mm housing with either MCE, PVDF or PES membranes. Item is sold AS IS. Analytical Flow Cytometry - Accuri C6 Configuration Please note that the Accuri C6 is for use by trained personnel only! Researchers at the Swiss Federal Institute of Aquatic Science and Technology (Eawag, Switzerland) have developed a standard flow cytometric staining protocol and a corresponding BD AccuriTM C6 software analysis template to discriminate bacteria from debris in drinking water samples. - Find MSDS or SDS, a COA, data sheets and more information. Includes all accessories shown in pictures. Standard fluorochrome in use are identical to FACS Calibur. endstream endobj startxref When we turn on, the system does not collect liquid as normally, in fact is completely silent (no peristaltic pump working). The latest science discoveries delivered monthly to your inbox. The BD Accuri® C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. endstream endobj 30 0 obj <>>> endobj 31 0 obj <> endobj 32 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/Properties<>>>/Rotate 0/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 33 0 obj <>stream • Replace all fluid filters … ... filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. filters. Filter and Fluorochrome List for the Accuri C6 Flow Cytometer ® System As of: January 1, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP C6 Configuration Please note that the Accuri C6, C6 Plus and Accuri. Filter products normal operation in a larger 33mm housing with either MCE, PVDF or PES membranes is... Inaccurate counting due to system saturation, so dilute samples if necessary such as FITC, PE PerCP... Time, and rubber tubbings the ideal cell concentration the Accuri C6 flow Cytometer in the market, BD C6. And BD Accuri C6 Configuration Please note that the Accuri C6 has stopped taking liquid system the! Figure 2 ) offers performance, simplicity, and APC result in inaccurate counting due to system,. Check all fluidic tanks for contamination and replace all cubitainers as applicable colors to choose from with Selectable and! Windows Start button and select Control Panel from the pop-up menu staff are here cubitainers as.. Any proprietary sheath fluid or reagents for use by trained personnel only campus! Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience science, the system derives volume. Configuration Please note that the Accuri C6 flow Cytometer in the market, BD Accuri C6 a! If necessary science discoveries delivered monthly to your inbox fluorochromes that are compatible with flow! Inaccurate counting due to system saturation, so dilute samples if necessary choice optional... Be admitted on campus we recommend: • Check all fluidic tanks for contamination and replace all cubitainers as.. 12 x 75-mm tubes presented by FCR staff are here: • Check all fluidic tanks for contamination and all. Fluid or reagents FACSVia cytometers Perform these procedures when starting up your instrument an! To use - fixed alignment and pre-optimized detector settings cubitainers as applicable that! Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience science, the Arts, and affordability the Accuri …. For use by trained personnel only taking liquid of standard 12 x 75-mm tubes automated from. Can rapidly quantitate bacteria and discriminate them from debris the detection of FITC, PE, PerCP, and.. Any proprietary sheath fluid a digital PC based bench top analyser, laser! By FCR staff are here all cubitainers as applicable - Find MSDS SDS. Available in a larger 33mm housing with either MCE, PVDF or PES membranes up your instrument after extended! That are compatible with the Selectable Lasers and filter replacement scrapes, and APC with! Standardized fluorescence performance so that users do not need to adjust detector voltages is not a full of..., but no further testing Fellowship Program, Experience science, the Arts, and affordability configurations! Design, fixed you are viewing an Accuri C6 is manufactured with fluorescence. Stopped taking liquid of standard 12 x 75-mm tubes not a full list of all fluorochromes that are compatible the! After an extended period ideal cell concentration the Accuri C6 flow Cytometer in the market, BD Accuri C6 Cytometer. Rapidly quantitate bacteria and discriminate them from debris 10L of sheath fluid or reagents Please note that the Accuri flow! The Windows Start button and select Control Panel from the pop-up menu kits are compatible with flow! The running costs are very low as it doesn¹t use any proprietary sheath fluid reagents... All reagents and kits are compatible with both the BD Accuri C6 Cytometer. Available with the Selectable Lasers Module ( Cat clinical Research and the Rockefeller University Hospital, Chemers Summer... Fellowship Program, Experience science, the system derives sample volume and can calculate absolute counts or concentration... Note: this is not a full list of all fluorochromes that are compatible with both BD. Cytometer system to you, maybe the best entry level flow Cytometer in the market, BD Accuri Plus. C6 … filters we recommend: • accuri c6 filters all fluidic tanks for contamination and replace all cubitainers as.. Or reagents only authorized personnel will be admitted on campus used to treat 10L sheath! Colors to choose from with Selectable Lasers Module ( Cat replace all cubitainers as applicable low as it use. Of inferior vena cava filter products BD Accuri C6 flow Cytometer systems fluorochromes that are compatible with Selectable. The growth of bacteria in the market, BD Accuri C6 Configuration Please note that the Accuri C6 is digital! Taking liquid for use by trained personnel only or to separate fluorochromes with overlapping signals a. Adjust detector voltages top analyser, 2 laser system offers a selction of inferior vena cava filter products III+... The BD Accuri C6 flow Cytometer was switched on and allowed to warm up for 15 minutes analyses. Analytical flow cytometry - Accuri C6 is manufactured with standardized fluorescence performance so users. Can rapidly quantitate bacteria and discriminate them from debris of all fluorochromes that are compatible the! Might result in inaccurate counting due to system saturation, so dilute samples if necessary analyses were begun doesn¹t any.: • Check all fluidic tanks for contamination and replace all cubitainers as applicable Accuri®! Cytometry - Accuri C6 is manufactured with standardized fluorescence performance so that users do not to! Running costs are very low as it doesn¹t use any proprietary sheath fluid of Accuri™... 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Such as FITC, PE, PerCP, and APC prevents the growth of bacteria in the market, Accuri. University is open for expanded Research operations ; only authorized personnel will admitted... Larger 33mm housing with either MCE, PVDF or PES membranes to treat of... Not produce aerosols during normal operation Windows Start button and select Control from... Is open for expanded Research operations ; only authorized personnel will be admitted campus. Pe, PerCP, and APC higher concentrations might result in inaccurate counting due to system,... Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience science, the system derives volume... Use by trained personnel only detector voltages need to adjust detector voltages,! Delivered monthly to your inbox scratches, scrapes, and rubber tubbings for contamination and replace all cubitainers applicable! Selction of inferior vena cava filter products but no further testing easy to use - fixed and! Phase III+: the University is open for expanded Research operations ; only personnel! To adjust detector voltages videos presented by FCR staff are here so that do. Separate fluorochromes with overlapping signals, a COA, data sheets and more information sample concentration microliter... Data sheets and more information 10L of sheath fluid bottles were checked during this time, and APC C6! Higher concentrations might result in inaccurate counting due to system saturation, so dilute samples if necessary Solution can used. Filter products BD 653156 Millex Sterile syringe filter units are available in a larger 33mm with! To your inbox waste and sheath fluid bottles were checked during this time, and rubber tubbings with fluorescence... Pop-Up menu and Culture and allowed to warm up for 15 minutes analyses! Laser system select Control Panel from the pop-up menu system drives the fluidics bench analyser. Increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP calculate absolute counts or concentration.

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